News:
Cathrine Grimes and Natasha Zachara will run a workshop at the ASBMB annual meeting (Orlando, FL). The goal of the workshop is to highlight new tools for studying glycans and to answer questions about how to approach Glycosciences problems. Check out Page 48 of ASBMB today (April, 2019)
Congratulation to Marissa Martinez Ph.D. - who has been recruited to Foghorn Therapeutics to study changes in protein complexes in cancer.
We welcome BCMB rotation Student Jasmin Zarb to the Zachara Lab (January 2019)
Critical observations that shaped our understanding of the function(s) of intracellular glycosylation (O-GlcNAc) is accepted in FEBS Letters (November, 2018).
Congratulations to Kekoa and Hailun, their paper O-GlcNAcylation is required for mutant KRAS-induced lung tumorigenesis is accepted in the Journal of Clinical Investigation (November, 2018)
We welcome undergraduate researchers Kian Croston and Fiddia Zahra to the Zachara Lab (September, 2018)
We welcome BC rotation student Danielle Smith to the Zachara Lab (September, 2018)
The Zachara Lab has been awarded an R01 to study the regulation of the enzymes which cycle O-GlcNAc (July, 2018)
The Zachara Lab has been awarded a U01 to develop tools to study O-GlcNAc (July, 2018)
Johns Hopkins University has been awarded a grant to train the next generation of Glycoscientist (June, 2018). Check out Immersive Training in the Glycosciences.
Bhargavi Narayanan (BC Grad student) joins the Zachara lab (May, 2018)
Congratulations to Walter, whose approach for studying the impact of O-GlcNAc on protein stability is online (April, 2018)
Congratulations to team Apte, their studies describing the impact of O-GlcNAc on acetaminophen are online (April, 2018)
Congratulations to Thiago Dias M.D. who graduated with his Ph.D.(February, 2018)
Congratulates to the Pandey Lab, their approach BioSite is online (December, 2017)
Congratulations to Jen Groves who graduated with her Ph.D. (October, 2017)
We welcome BC rotation student Vincent Guo to the lab (October, 2017)
We welcome BC rotation student Bhargavi Narayanan to the lab (August, 2017)
Congratulations to Jen Groves, whose paper “Characterization of tools to detect and enrich human and mouse O-GlcNAcase ” is in press in Glycobiology (June, 2017)
Congratulations Team Slawson - their paper describing roles for O-GlcNAc in regulating mitochondrial function is online (July, 2017)
Look out for Transforming Glycoscience, news about the common fund whose goal is to develop transformative tools to help researchers study the roles of glycans in diverse physiological processes (May, 2017).
Congratulation Austin, who will be attending DUKE in the fall to complete his M.D./Ph.D.
Congratulations to Jen Groves, whose paper “Fatty Acid Synthase Inhibits O-GlcNAcase During Oxidative Stress” is in press in the Journal of Biological Chemistry (February, 2017)
Congratulations Marissa, Peter and Thiago, whose review “Stress-Induced O-GlcNAcylation, an Adaptive Process of Injured Cells” is in press in Biochemical Society Transactions (December, 2017).
Congratulations to Jen and Marissa who have won Travel Awards to present their work at the Society for Glycobiology Annual Meeting (November, 2016).
Congratulations to Albert, whose paper “Combined Antibody/Lectin Enrichment Identifies Extensive Changes in the O-GlcNAc Subproteome Upon Oxidative Stress” is in press at the Journal of Proteome Research (September, 2016)
Austin has been awarded the 2016 Marion B. Sewer Distinguished Scholarship by the American Society for Biochemistry and Molecular Biology (ASBMB). Congratulations!! (August 2016).
Congratulations to Kamau, whose review “Molecular Functions of Glycoconjugates in Autophagy” was accepted by the Journal of Molecular Biology (June, 2016).
Congratulations to Kekoa, whose review “Hijacking the Hexosamine Biosynthetic Pathway to Promote EMT-Mediated Neoplastic Phenotypes” was accepted by Frontiers in Endocrinology (April, 2016).
Congratulations to Peter Natov, who has accepted an offer to complete his degree in medicine at Tufts University School of Medicine (May, 2016)
Congratulations Sandy! Her paper Identification and biological consequences of the O-GlcNAc modification of the human innate immune receptor, Nod2 was accepted in the Glycobiology. Journal (2015)
The Zachara Lab was awarded a common fund grant to develop “Chemical genetic tools for the spatial and temporal modulation of O-GlcNAcylation” (July, 2015).
Congratulations to Yanping, whose paper “Post-translational O-GlcNAcylation is essential for nuclear pore integrity and maintenance of the pore selectivity filter” has been accepted by the Journal of Molecular Cell Biology (June, 2015).
Congratulations to Roger Henry, who has accepted an offer to complete his degree in medicine at Rutgers Robert Wood Johnson Medical School in 2016 (May, 2015).
Publications:
Sager RA, Woodford MR, Backe SJ, Makedon AM, Baker-Williams AJ, DiGregorio BT, Loiselle DR, Haystead TA, Zachara NE, Prodromou C, Bourboulia D, Schmidt LS, Linehan WM, Bratslavsky G, Mollapour M. Post-translational Regulation of FNIP1 Creates a Rheostat for the Molecular Chaperone Hsp90.Cell Rep. 2019 Jan 29;26(5):1344-1356.e5. PMID:
30699359
Zachara NE. Critical observations that shaped our understanding of the function(s) of intracellular glycosylation (O-GlcNAc). FEBS Lett. 2018 Dec; 592(23):3950-3975. PMID: 30414174
Taparra K, Wang H, Malek R, Lafargue A, Barbhuiya MA, Wang X, Simons BW, Ballew M, Nugent K, Groves J, Williams RD, Shiraishi T, Verdone J, Yildirir G, Henry R, Zhang B, Wong J, Wang KK, Nelkin BD, Pienta KJ, Felsher D, Zachara NE*, Tran PT*. O-GlcNAcylation is required for mutant KRAS-induced lung tumorigenesis. J Clin Invest. 2018 128(11):4924-4937. *Co-corresponding authors. PMID: 30130254
Drake WR, Hou CW, Zachara NE, Grimes CL. New use for CETSA: monitoring innate immune receptor stability via post-translational modification by OGT. J Bioenerg Biomembr. 2018;50(3):231-240. PMID: 29671171
Sack GH Jr, Zachara N, Rosenblum N, Talbot CC Jr, Kreimer S, Cole R, McDonald TL. Serum amyloid A1 (SAA1) protein in human colostrum. FEBS Open Bio. 2018;8(3):435-441. PMID:29511620
McGreal SR, Bhushan B, Walesky C, McGill MR, Lebofsky M, Kandel SE, Winefield RD, Jaeschke H, Zachara NE, Zhang Z, Tan EP, Slawson C, Apte U. Modulation of O-GlcNAc levels in the liver impacts acetaminophen-induced liver injury by affecting protein adduct formation and glutathione synthesis. Toxicol Sci., 2018; 162(2):599-610. https://www.ncbi.nlm.nih.gov/pubmed/29325178
Kim DI, Cutler JA, Na CH, Reckel S, Renuse S, Madugundu AK, Tahir R, Goldschmidt HL, Reddy KL, Huganir RL, Wu X, Zachara NE, Hantschel O, Pandey A. BioSITe: A Method for Direct Detection and Quantitation of Site-Specific Biotinylation. J Proteome Res. 2018;17(2):759-769. PMID:29249144
Zachara NE, Hart GW, and Akimoto, Y. The O-GlcNAc Modification. In Essentials in Glycobiology, 3rd Edition. Cold Spring Harbor (NY). Chapter 19, 2015-2017. PMID: 28876858
Tan EP, McGreal SR, Graw S, Tessman R, Koppel SJ, Dhakal P, Zhang Z, Machacek M, Zachara NE, Koestler DC, Peterson KR, Thyfault JP, Swerdlow RH, Krishnamurthy P, DiTacchio L, Apte U, Slawson C. Sustained O-GlcNAcylation reprograms mitochondrial function to regulate energy metabolism. J Biol. Chem. 2017; 292(36):14940-14962. PMID: 28739801
Groves JA and Zachara NE. Characterization of tools to detect and enrich human and mouse O-GlcNAcase. Glycobiology, In Press. PMID: 28595377
Groves JA, Maduka AO, O’Meally RN, Cole RN, and Zachara NE.. Fatty acid synthase: a novel oxidative stress-induced interactor and inhibitor of the O-GlcNAcase. JBC, 2017; 292(16):6493-6511. PMID: 28232487.
Martinez M, Dias T, Natov P, and Zachara NE. Stress-Induced O-GlcNAcylation, an Adaptive Process of Injured Cells. Biochemical Society Transactions, 2017; 45(1): 237-249. PMID: 28202678.
Lee A, Henry R, Miller D, Paruchuri VDP, O’Meally R, Boronina T, Cole RN, Zachara NE. Combined Antibody/Lectin-Enrichment Identifies Extensive Changes in the O-GlcNAc Subproteome Upon Oxidative Stress, Journal of Proteome Research; 15(12):4318-4336. PMID: 27669760
Fahie K, Zachara NE. Molecular Functions of Glycoconjugates in Autophagy. J Mol Biol. 2016; 428(16):3305-3024. Review. PMID: 27345664
Taparra K, Tran PT, Zachara NE. Hijacking the Hexosamine Biosynthetic Pathway to Promote EMT-Mediated Neoplastic Phenotypes. Front Oncol. 2016 Apr 18;6:85. PMID:27148477
Hou CW, Mohanan V, Zachara NE, Grimes CL. Identification and biological consequences of the O-GlcNAc modification of the human innate immune receptor, Nod2. Glycobiology. 2016; 26(1):13-18. PMID: 26369908.
Zhu Y, Liu TW, Madden Z, Yuzwa SA, Murray K, Cecioni S, Zachara N, Vocadlo DJ. (2015) Post-translational O-GlcNAcylation is essential for nuclear pore integrity and maintenance of the pore selectivity filter. J Mol Cell Biol., 2015; 8(1):2-16. PMID: 26031751. PUBMED
Zhong J., Martinez M., Sengupta S., Lee A., Wu X., Chaerkady R., O'Meally R., Cole R. N., Pandey A., Zachara N. E. Quantitative Phosphoproteomics Reveals Crosstalk Between Phosphorylation and O-GlcNAc in the DNA Damage Response Pathway. Proteomics, 15(2-3):591-607. PUBMED
R.R. Reeves, A. Lee, R. Henry and N.E. Zachara. (2014) Characterization of the specification of O-GlcNAc reactive antibodies under conditions of starvation and stress. 457: 8-18. PUBMED.
Lidia Tardio*, Jessica Andrés-Bergós*, Natasha E. Zachara, Ane Larrañaga-Vera, Camino Rodriguez-Villar, Gabriel Herrero-Beaumont, Raquel Largo. O-Linked N-acetylglucosamine (O-GlcNAC) Protein Modification is Increased in the Cartilage of Patients with Knee Osteoarthritis. 22(2): 259-63, Osteoarthritis and Cartilage. PUBMED Denotes Co-First Authors
J.A. Groves, A. Lee, G. Yildirir, N.E. Zachara (2013) Dynamic O-GlcNAcylation and its roles in the cellular stress response and homeostasis. Cells Stress and Chaperones. 2013, 18(5): 535-58. PUMBED* Denotes Co-First Authors
R.V. Jensesn, J. Johnsen, S.B. Kristiansen, N.E. Zachara and H.E. Botker. Ischemic Preconditioning Increases Myocardial O-GlcNAc Glycosylation. Scandinavian Cardiovascular Journal, 47(3): 168-74. PUBMED.
R.V. Jensen, N.E. Zachara, P.H. Nielsen, H.H. Kimose, S.B. Kristiansen, and H.E. Botker. Impact of O-GlcNAc on cardioprotection by remote ischemic preconditioning in non-diabetic and diabetic patients. Cardiovascular Research, 2013, 97(2): 369-78. PUBMED
M.K. Tarrant, H. Rho, Z. Xie, Y. Jiang, C. Gross, J. Qian, Y. Ichikawa, N. Zachara, F. Etzkorn, G. W. Hart, J. Jeong, S. Blackshaw, H. Zhu, P. A. Cole. Multi-faceted Regulation of Protein Kinase CK2 by Phosphorylation and Glycosylation Revealed through Semisynthesis. Nature Chemical Biology, 2012 Jan 22;8(3):262-9. PUBMED
N.E. Zachara The Roles of O-Linked b-N-Acetylglucosamine (O-GlcNAc) in Cardiac Physiology and Disease. AJP m J Physiol Heart Circ Physiol. 2012 May;302(10):H1905-18. PUBMED
V.D.P. Paruchuri and N. E. Zachara. “Defining the Cardiac O-GlcNAcome, A Review of Approaches and Methadologies”, Special series: Integrating proteomics into cardiovascular disease in Circulation: Cardiovascular Genetics, 2011 Dec;4(6):710. PUBMED
N. E. Zachara Keith L Vosseller, and G. W. Hart (2011) Detection and analysis of proteins modified by O-Linked N-acetylglucosamine. In Current Protocols in Protein Science, Johns Wiley and Sons Inc., New York, USA. Chapter 12, Unit 12.8. PUBMED
N. E. Zachara Keith L Vosseller, and G. W. Hart (2011) Detection and analysis of proteins modified by O-Linked N-acetylglucosamine. In Curr Protoc Mol Biol Johns Wiley and Sons Inc., New York, USA. Chapter 17:Unit 17.6. PUBMED
N.E. Zachara, H. Molina, K. Wong, A. Pandey, G.W. Hart (2011) The dynamic stress-induced O-GlcNAcome highlights functions for O-GlcNAc in DNA Repair and other cellular pathways. Amino Acids 40(3): 793-808. PUBMED
Z. Kazemi, H. Chang, S. K. Haserodt, C. McKen, N.E. Zachara (2010) O-GlcNAc Regulates Stress-Induced Heat Shock Protein Expression in a GSK-3b Dependent Manner. J. Biol. Chem. 285(50):39096-107. PUBMED
N. E. Zachara (2009) Detection and Analysis of Nuclearcytoplasmic Glycoproteins. In The Nucleus: Principles and Protocols, Meth Mol Biol. Hancock R (ed) Humana Press (Totowa, USA). Volume 464, pp. 227-254. PUBMED
N. E. Zachara (2009) Detecting the “O-GlcNAc-ome”; Detection, Purification, and Analysis of O-GlcNAc Modified Proteins. In Glycomics, Meth Mol Biol. Packer N, Karlsson GN (eds) Humana Press (Totowa, USA). Volume 534, pp.251-79. PUBMED
G. A. Ngoh, H. T. Facundo, T. Hamid, W. Dillmann, N. E. Zachara, Steven P. Jones (2009). Unique Hexosaminidase Reduces Metabolic Survival Signal and Sensitizes Cardiac Myocytes to Hypoxia-Reoxygenation Injury. Circ. Res. 104(1):41-9. PUBMED
S. P. Jones, N. E. Zachara, Y. Teshima, G. W. Hart, and E. Marban. (2008) Endogenously-recruitable cardioprotection by N-acetylglucosamine linkage to cellular proteins. Circulation, 117(9):1172-82. PUBMED
N.E. Zachara. (2007) The sweet nature of cardioprotection. Amer J Phys (Heart and Circulatory Physiology; 293: H1324-H1326. PUBMED
N. E. Zachara and G. W. Hart. (2006) Cell Signaling, the Essential Role of O-GlcNAc! Biochim. Biophys. Acta, 1761: 599-617. PUBMED
C. Slawson, N. E. Zachara, K. Vosseller, W. Cheung, M. D. Lane, and G. W. Hart (2005) Perturbations in O-GlcNAc protein modification cause severe defects in mitotic progression and cytokinesis. J. Biol. Chem., 280, 32944-32956. PUBMED
N. E. Zachara and G. W. Hart. (2004) O-GlcNAc a sensor of cellular state: The role of nucleocytoplasmic glycosylation in modulating cellular function in response to nutrition and stress. Biochim. Biophys. Acta., 1673, 13-28. PUBMED
N. E. Zachara, N. O'Donnell, J. J. Mercer, J. D. Marth, and G. W. Hart. (2004) Dynamic O-GlcNAc modification of nucleocytoplasmic proteins in response to stress. A survival response of mammalian cells. J. Biol. Chem., 279, 30133-30142. PUBMED
N. O’Donnell, N. Zachara, G. W. Hart, and J. D. Marth. (2004) OGT-dependent X-chromosome-linked intracellular protein glycosylation is essential for mammalian viability and cellular metabolism. Mol. Cell. Biol., 24, 1680-1690. PUBMED
C. Gewinner, G. W. Hart, N. E. Zachara, R. N. Cole, C. Beisenherz-Huss, and B. Groner. (2004) The coactivator of transcription CREB binding protein interacts preferentially with the glycosylated form of Stat5. J. Biol. Chem., 279, 3563-3572. PUBMED
N. E. Zachara, W. D. Cheung, and G. W. Hart (2004). O-GlcNAc, an essential post-translational modification of nucleocytoplasmic proteins. Current Organic Chemistry, 8, 369-383.
N. E. Zachara, W. D. Cheung, and G. W. Hart. (2004) The detection and analysis of nucleocytoplasmic protein glycosylation, O-GlcNAc. In Signal Transduction Protocols, Methods in Molecular Biology, R. Dickson and M. Mendenhall (eds), Humana Press, Totowa, USA. Volume 284, pp.175-194. PUBMED
N. E. Zachara and G. W. Hart. (2004) Protein glycosylation. In The Encyclopedia of Biochemistry, W. Lennarz and M. D. Lane (eds), Academic Press/Elsevier Science, New York, USA.Volume 3: 504-509.
N. E. Zachara and G. W. Hart. (2004) O-GlcNAc, a nutritional effector molecule, modulates proteasome function. Trends in Cell Biology, 14, 218-221. PUBMED
N. E. Zachara and G. W. Hart. (2002) The functional significance of O-GlcNAc in cellular processes. Chemical Reviews, 102, 431-438. PUBMED
N. E. Zachara, R. N. Cole, G. W. Hart, and Y. Gao. (2002) Detection and analysis of proteins modified by O-Linked N-acetylglucosamine. In Current Protocols in Molecular Biology, Johns Wiley and Sons Inc., New York, USA. Chapter 17, pp17.6.1-17.6.22. PUBMED
N. E. Zachara, R. N. Cole, G. W. Hart, and Y. Gao. (2001) Detection and analysis of proteins modified by O-Linked N-acetylglucosamine. In Current Protocols in Protein Science, Johns Wiley and Sons Inc., New York, USA. Chapter 12, Unit 12.8, pp12.8.1-12.8.25. PUBMED
N. E. Zachara and A. A. Gooley. (2000) Identification of glycosylation sites in mucin peptides. In Mucin Methods and Protocols, Methods in Molecular Biology, T. Corfield (ed) Humana Press, Totowa, USA. 125, 121-128. PUBMED
T. Wugeditsch, N. E. Zachara, M. Puchberger, P. Kosma, A. A. Gooley, and P. Messner. (1999) Structural heterogeneity in the core oligosaccharide of the S-layer glycoprotein from Aneurinibacillus thermoaerophilus DSM 10155. Glycobiology, 9, 787-795. PUBMED
S. Muller, K. Alving, J. Peter-Katalinic, N. E. Zachara, A. A. Gooley, F. G. and Hanisch. (1999) High density O-Glycosylation on tandem repeat peptide from secretory MUC1 of T47D breast cancer cells. J. Biol. Chem., 274, 18165-18172. PUBMED
F. G. Hanisch, S. Muller, H. Hassan, H. Clausen, N. E. Zachara, A. A. Gooley, H. Paulsen, K. Alfing, and J. Peter-Katalinic. (1999) Dynamic epigenetic regulation of initial O-glycosylation by UDP-N-Acetylgalactosamine:peptide N-acetylgalactosaminyl transferases. Site-specific glycosylation of MUC1 repeat peptide influences the substrate qualities at adjacent or distant Ser/Thr positions. J. Biol. Chem., 274, 9946-9954. PUBMED
M. J. Harrison, A. S. Nouwens, D. Jardine, N. E. Zachara, H. Nevalainen, A. A. Gooley, and N. H. Packer. (1998) Identification of unusual post-translational modifications of cellobiohydrolase I from Trichoderma Reesei. Eur. J. Biochem., 256, 119-217. PUBMED
H. Nevalainen, M. Harrison, D. Jardine, N. E. Zachara, M. Paloheimo, P. Suominen, A. A. Gooley, and N. Packer. (1997) Glycosylation of cellobiohydrolases from Trichoderma reesei. carbohydrates from Trichoderma reesei and other microorgansims: structure, biochemistry, genetics and applications. The Royal Society of Chemistry, Thomas Graham House, Cambridge, UK. pp 335-344.
N. E. Zachara, N. H. Packer, M. D. Temple, M. B. Slade, D. R. Jardine, P. Karuso, C. J. Moss, B. C. Mabbutt, P. M. G. Curmi, K. L. Williams, and A. A. Gooley. (1996) Recombinant Prespore-specific Antigen from Dictyostelium Discoideum is a -Sheet glycoprotein with a spacer peptide modified by O-linked N-acetylglucosamine. Eur. J. Biochem., 238 511-518. PUBMED