Zachara Lab

4-hydroxytamoxifen (500uM): typically this is a 1000x stock
  • 5mg 4-hydroxytamoxifen (Sigma H7904, MW=387.5)
  • Dissolve in 25mls of ethanol (200 proof)
  • Aliquot in glass vials
  • Store at -20oC in the dark at -20oC
PIC2 (Protease inhibitor cocktail 2; Calbiochem #539132)
  • Reconstitute each vial of lyophilized protease inhibitor cocktail with 1 ml of DMSO first.
  • Mix gently.
  • Then add 4 ml of water.
  • The solution will appear turbid.
  • Following reconstitution, aliquot and freeze at -20°C. The reconstituted material is stable for up to 6 months at -20°C.
  • This is a 1000X concentrate.