Checkout common protocols and tools used by the Zachara Lab in linked pages for:


  • Common Protocols
  • O-GlcNAc Immunoblotting
  • OGT Inducible Null Cells
  • Our Favorite Non-Lab Recipes (We love to bake)

O-GlcNAc Protocol Publications:


Fahie K, Narayanan B, Zahra F, Reeves R, Fernandes SM, Hart GW, Zachara NE. Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine. Curr Protoc. 2021 May;1(5):e129. doi: 10.1002/cpz1.129. PMID: 34004049; PMCID: PMC8862748.

Reeves RA, Lee A, Henry R, Zachara NE. Characterization of the specificity of O-GlcNAc reactive antibodies under conditions of starvation and stress. Anal Biochem. 2014 Jul 15;457:8-18. doi: 10.1016/j.ab.2014.04.008. Epub 2014 Apr 18. PMID: 24747005; PMCID: PMC4113912.

Zachara NE, Vosseller K, Hart GW. Detection and analysis of proteins modified by O-linked N-acetylglucosamine. Curr Protoc Protein Sci. 2011 Nov;Chapter 12:12.8.1-12.8.33. doi: 10.1002/0471140864.ps1208s66. PMID: 22045558; PMCID: PMC3349994.

Zachara NE, Vosseller K, Hart GW. Detection and analysis of proteins modified by O-linked N-acetylglucosamine. Curr Protoc Mol Biol. 2011 Jul;Chapter 17:Unit 17.6. doi: 10.1002/0471142727.mb1706s95. PMID: 21732316; PMCID: PMC3329785.

Zachara NE. Detecting the "O-GlcNAc-ome"; detection, purification, and analysis of O-GlcNAc modified proteins. Methods Mol Biol. 2009;534:251-79. doi: 10.1007/978-1-59745-022-5_19. PMID: 19277546.

Zachara NE. Detection and analysis of (O-linked beta-N-acetylglucosamine)-modified proteins. Methods Mol Biol. 2009;464:227-54. doi: 10.1007/978-1-60327-461-6_13. PMID: 18951188.

Zachara NE, Cheung WD, Hart GW. Nucleocytoplasmic glycosylation, O-GlcNAc: identification and site mapping. Methods Mol Biol. 2004;284:175-94. doi: 10.1385/1-59259-816-1:175. PMID: 15173616.

Zachara NE, Hart GW, Cole RN, Gao Y. Detection and analysis of proteins modified by O-linked N-acetylglucosamine. Curr Protoc Mol Biol. 2002 Feb;Chapter 17:Unit 17.6. doi: 10.1002/0471142727.mb1706s57. PMID: 18265305.

Zachara NE, Cole RN, Hart GW, Gao Y. Detection and analysis of proteins modified by O-linked N-acetylglucosamine. Curr Protoc Protein Sci. 2001 Nov;Chapter 12:Unit 12.8. doi: 10.1002/0471140864.ps1208s25. PMID: 18429112.