We have generated a cell line in which OGT can be deleted upon addition of 4-hydroxytamoxifen (Kazemi et al., 2010). We are happy to share this cell line and provide a detailed protocol for using these cells. Please accompany your request with the following MTA questionnaire, which can be downloaded here. Note: your request should be sent to Dr Zachara ([email protected]). Requests for the MEFs carrying the LoxP flanked OGT gene should be made to Jamey Marth (UCSD).
Antibodies used to detect O-GlcNAc
Links to Protocol Chapters we have written
N. E. Zachara Keith L Vosseller, and G. W. Hart (2011) Detection and analysis of proteins modified by O-Linked N-acetylglucosamine. In Current Protocols in Protein Science, Johns Wiley and Sons Inc., New York, USA. Chapter 12, Unit 12.8. PUBMED
N. E. Zachara Keith L Vosseller, and G. W. Hart (2011) Detection and analysis of proteins modified by O-Linked N-acetylglucosamine. In Curr Protoc Mol Biol Johns Wiley and Sons Inc., New York, USA. Chapter 17:Unit 17.6. PUBMED
N. E. Zachara (2009) Detection and Analysis of Nuclearcytoplasmic Glycoproteins. In The Nucleus: Principles and Protocols, Meth Mol Biol. Hancock R (ed) Humana Press (Totowa, USA). Volume 464, pp. 227-254. PUBMED
N. E. Zachara (2009) Detecting the “O-GlcNAc-ome”; Detection, Purification, and Analysis of O-GlcNAc Modified Proteins. In Glycomics, Meth Mol Biol. Packer N, Karlsson GN (eds) Humana Press (Totowa, USA). Volume 534, pp.251-79. PUBMED
N. E. Zachara, W. D. Cheung, and G. W. Hart. (2004) The detection and analysis of nucleocytoplasmic protein glycosylation, O-GlcNAc. In Signal Transduction Protocols, Methods in Molecular Biology, R. Dickson and M. Mendenhall (eds), Humana Press, Totowa, USA. Volume 284, pp.175-194. PUBMED