Publications

2024

Umapathi P, Aggarwal A, Zahra F, Narayanan B, Zachara NE. The Multifaceted Role of Intracellular Glycosylation in Cytoprotection and Heart Disease. J Biol Chem. 2024 Apr 17:107296. doi: 10.1016/j.jbc.2024.107296. Epub ahead of print. PMID: 38641064.

Buchner J, Alasady MJ, Backe SJ, Blagg BSJ, Carpenter RL, Colombo G, Gelis I, Gewirth DT, Gierasch LM, Houry WA, Johnson JL, Kang BH, Kao AW, LaPointe P, Mattoo S, McClellan AJ, Neckers LM, Prodromou C, Rasola A, Sager RA, Theodoraki MA, Truman AW, Truttman MC, Zachara NE, Bourboulia D, Mollapour M, Woodford MR. Second international symposium on the chaperone code, 2023. Cell Stress Chaperones. 2024 Feb;29(1):88-96. doi: 10.1016/j.cstres.2024.01.003. Epub 2024 Feb 3. PMID: 38316354; PMCID: PMC10939070.

2023

Narayanan B, Zachara NE. Regulation of Cell Physiology by O-GlcNAc. Encyclopedia of Cell Biology, 2nd edition. Editor(s): Bradshaw RA, Hart GW, Stahl PD.
Academic Press, 2023; 4: 309-19. https://doi.org/10.1016/B978-0-12-821618-7.00015-8.Elsevier (Amsterdam, Netherlands), In press.

Narayanan B, Sinha P, Henry R, Reeves RA, Paolocci N, Kohr MJ, Zachara NE. Cardioprotective O-GlcNAc signaling is elevated in murine female hearts via enhanced O-GlcNAc transferase activity. J Biol Chem. 2023 Dec;299(12):105447. doi: 10.1016/j.jbc.2023.105447. Epub 2023 Nov 8. PMID: 37949223; PMCID: PMC10711226.

Robarts DR, Kotulkar M, Paine-Cabrera D, Venneman KK, Hanover JA, Zachara NE, Slawson C, Apte U. The essential role of O-GlcNAcylation in hepatic differentiation. Hepatol Commun. 2023 Nov 6;7(11):e0283. doi: 10.1097/HC9.0000000000000283. PMID: 37930118; PMCID: PMC10629742.

Narayanan B, Zahra F, Reeves RA, Aggarwal A, O'Meally RN, Henry RK, Craven M, Jacobson A, Cole RN, Kohr MJ, Umapathi P, Zachara NE. Differential Detection of O-GlcNAcylated proteins in the heart using antibodies. Anal Biochem. 2023 Oct 1;678:115262. doi: 10.1016/j.ab.2023.115262. Epub 2023 Jul 27. PMID: 37507081; PMCID: PMC10523836.

Mishra S, Ma J, McKoy D, Sasaki M, Farinelli F, Page RC, Ranek MJ, Zachara N, Kass DA. Transient receptor potential canonical type 6 (TRPC6) O-GlcNAcylation at Threonine-221 plays potent role in channel regulation. iScience. 2023 Feb 28;26(3):106294. doi: 10.1016/j.isci.2023.106294. PMID: 36936781; PMCID: PMC10014292.

Lewis AL, Toukach P, Bolton E, Chen X, Frank M, Lütteke T, Knirel Y, Schoenhofen I, Varki A, Vinogradov E, Woods RJ, Zachara N, Zhang J, Kamerling JP, Neelamegham S; SNFG Discussion Group. Cataloging natural sialic acids and other nonulosonic acids (NulOs), and their representation using the Symbol Nomenclature for Glycans. Glycobiology. 2023 Mar 6;33(2):99-103. doi: 10.1093/glycob/cwac072. PMID: 36648443; PMCID: PMC9990982.

Papanicolaou KN, Jung J, Ashok D, Zhang W, Modaressanavi A, Avila E, Foster DB, Zachara NE, O'Rourke B. Inhibiting O-GlcNAcylation impacts p38 and Erk1/2 signaling and perturbs cardiomyocyte hypertrophy. J Biol Chem. 2023 Mar;299(3):102907. doi: 10.1016/j.jbc.2023.102907. Epub 2023 Jan 13. PMID: 36642184; PMCID: PMC9988579.

2022

Fahie KMM, Papanicolaou KN, Zachara NE. Integration of O-GlcNAc into Stress Response Pathways. Cells. 2022 Nov 5;11(21):3509. doi: 10.3390/cells11213509. PMID: 36359905; PMCID: PMC9654274.

Robarts DR, McGreal SR, Umbaugh DS, Parkes WS, Kotulkar M, Abernathy S, Lee N, Jaeschke H, Gunewardena S, Whelan SA, Hanover JA, Zachara NE, Slawson C, Apte U. Regulation of Liver Regeneration by Hepatocyte O-GlcNAcylation in Mice. Cell Mol Gastroenterol Hepatol. 2022;13(5):1510-1529. doi: 10.1016/j.jcmgh.2022.01.014. Epub 2022 Jan 28. PMID: 35093590; PMCID: PMC9043307.

West CM, Slawson C, Zachara NE, Hart GW. Nucleocytoplasmic Glycosylation. In: Varki A, Cummings RD, Esko JD, Stanley P, Hart GW, Aebi M, Mohnen D, Kinoshita T, Packer NH, Prestegard JH, Schnaar RL, Seeberger PH, editors. Essentials of Glycobiology [Internet]. 4th ed. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2022. Chapter 18. PMID: 35536974.

Zachara NE, Akimoto Y, Boyce M, Hart GW. The O-GlcNAc Modification. In: Varki A, Cummings RD, Esko JD, Stanley P, Hart GW, Aebi M, Mohnen D, Kinoshita T, Packer NH, Prestegard JH, Schnaar RL, Seeberger PH, editors. Essentials of Glycobiology [Internet]. 4th ed. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2022. Chapter 19. PMID: 35536957.

Freeze HH, Boyce M, Zachara NE, Hart GW, Schnaar RL. Glycosylation Precursors. In: Varki A, Cummings RD, Esko JD, Stanley P, Hart GW, Aebi M, Mohnen D, Kinoshita T, Packer NH, Prestegard JH, Schnaar RL, Seeberger PH, editors. Essentials of Glycobiology [Internet]. 4th ed. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 2022. Chapter 5. PMID: 35536946.

2021

Costa TJ, Potje SR, Fraga-Silva TFC, da Silva-Neto JA, Barros PR, Rodrigues D, Machado MR, Martins RB, Santos-Eichler RA, Benatti MN, de Sá KSG, Almado CEL, Castro ÍA, Pontelli MC, Serra L, Carneiro FS, Becari C, Louzada-Junior P, Oliveira RDR, Zamboni DS, Arruda E, Auxiliadora-Martins M, Giachini FRC, Bonato VLD, Zachara NE, Bomfim GF, Tostes RC. Mitochondrial DNA and TLR9 activation contribute to SARS-CoV-2-induced endothelial cell damage. Vascul Pharmacol. 2022 Feb;142:106946. doi: 10.1016/j.vph.2021.106946. Epub 2021 Nov 25. PMID: 34838735; PMCID: PMC8612754.

Fahie K, Narayanan B, Zahra F, Fernandes SM, Zachara NE. Transforming Technologies to Advance Our Understanding of the Functions of O-GlcNAc. In Barchi Jr., Joseph (Ed). Comprehensive Glycoscience (2nd edition). Volume 3, pp288-302. Elsivier (Oxford).

Fahie K, Narayanan B, Zahra F, Reeves R, Fernandes SM, Hart GW, Zachara NE. Detection and Analysis of Proteins Modified by O-Linked N-Acetylglucosamine. Curr Protoc. 2021 May;1(5):e129. doi: 10.1002/cpz1.129. PMID: 34004049; PMCID: PMC8862748.

Martinez M, Renuse S, Kreimer S, O'Meally R, Natov P, Madugundu AK, Nirujogi RS, Tahir R, Cole R, Pandey A, Zachara NE. Quantitative Proteomics Reveals that the OGT Interactome Is Remodeled in Response to Oxidative Stress. Mol Cell Proteomics. 2021;20:100069. doi: 10.1016/j.mcpro.2021.100069. Epub 2021 Mar 12. PMID: 33716169; PMCID: PMC8079276.

Umapathi P, Mesubi OO, Banerjee PS, Abrol N, Wang Q, Luczak ED, Wu Y, Granger JM, Wei AC, Reyes Gaido OE, Florea L, Talbot CC Jr, Hart GW, Zachara NE, Anderson ME. Excessive O-GlcNAcylation Causes Heart Failure and Sudden Death. Circulation. 2021 Apr 27;143(17):1687-1703. doi: 10.1161/CIRCULATIONAHA.120.051911. Epub 2021 Feb 17. Erratum in: Circulation. 2021 Apr 27;143(17):e892. PMID: 33593071; PMCID: PMC8085112.

Levine ZG, Potter SC, Joiner CM, Fei GQ, Nabet B, Sonnett M, Zachara NE, Gray NS, Paulo JA, Walker S. Mammalian cell proliferation requires noncatalytic functions of O-GlcNAc transferase. Proc Natl Acad Sci U S A. 2021 Jan 26;118(4):e2016778118. doi: 10.1073/pnas.2016778118. PMID: 33419956; PMCID: PMC7848692.

Mesubi OO, Rokita AG, Abrol N, Wu Y, Chen B, Wang Q, Granger JM, Tucker-Bartley A, Luczak ED, Murphy KR, Umapathi P, Banerjee PS, Boronina TN, Cole RN, Maier LS, Wehrens XH, Pomerantz JL, Song LS, Ahima RS, Hart GW, Zachara NE, Anderson ME. Oxidized CaMKII and O-GlcNAcylation cause increased atrial fibrillation in diabetic mice by distinct mechanisms. J Clin Invest. 2021 Jan 19;131(2):e95747. doi: 10.1172/JCI95747. PMID: 33151911; PMCID: PMC7810480.

2019

Sager RA, Woodford MR, Backe SJ, Makedon AM, Baker-Williams AJ, DiGregorio BT, Loiselle DR, Haystead TA, Zachara NE, Prodromou C, Bourboulia D, Schmidt LS, Linehan WM, Bratslavsky G, Mollapour M. Post-translational Regulation of FNIP1 Creates a Rheostat for the Molecular Chaperone Hsp90.Cell Rep. 2019 Jan 29;26(5):1344-1356.e5. PMID: 30699359

2018

Zachara NE. Critical observations that shaped our understanding of the function(s) of intracellular glycosylation (O-GlcNAc). FEBS Lett. 2018 Dec; 592(23):3950-3975. PMID: 30414174

Taparra K, Wang H, Malek R, Lafargue A, Barbhuiya MA, Wang X, Simons BW, Ballew M, Nugent K, Groves J, Williams RD, Shiraishi T, Verdone J, Yildirir G, Henry R, Zhang B, Wong J, Wang KK, Nelkin BD, Pienta KJ, Felsher D, Zachara NE*, Tran PT*. O-GlcNAcylation is required for mutant KRAS-induced lung tumorigenesis. J Clin Invest. 2018 128(11):4924-4937. *Co-corresponding authors. PMID: 30130254

Drake WR, Hou CW, Zachara NE, Grimes CL. New use for CETSA: monitoring innate immune receptor stability via post-translational modification by OGT. J Bioenerg Biomembr. 2018;50(3):231-240. PMID: 29671171

Sack GH Jr, Zachara N, Rosenblum N, Talbot CC Jr, Kreimer S, Cole R, McDonald TL. Serum amyloid A1 (SAA1) protein in human colostrum. FEBS Open Bio. 2018;8(3):435-441. PMID:29511620

McGreal SR, Bhushan B, Walesky C, McGill MR, Lebofsky M, Kandel SE, Winefield RD, Jaeschke H, Zachara NE, Zhang Z, Tan EP, Slawson C, Apte U. Modulation of O-GlcNAc Levels in the Liver Impacts Acetaminophen-Induced Liver Injury by Affecting Protein Adduct Formation and Glutathione Synthesis. Toxicol Sci. 2018 Apr 1;162(2):599-610. doi: 10.1093/toxsci/kfy002. PMID: 29325178; PMCID: PMC6012490.

Kim DI, Cutler JA, Na CH, Reckel S, Renuse S, Madugundu AK, Tahir R, Goldschmidt HL, Reddy KL, Huganir RL, Wu X, Zachara NE, Hantschel O, Pandey A. BioSITe: A Method for Direct Detection and Quantitation of Site-Specific Biotinylation. J Proteome Res. 2018;17(2):759-769. PMID:29249144

2017

Zachara NE, Hart GW, and Akimoto, Y. The O-GlcNAc Modification. In Essentials in Glycobiology, 3rd Edition. Cold Spring Harbor (NY). Chapter 19, 2015-2017. PMID: 28876858

Tan EP, McGreal SR, Graw S, Tessman R, Koppel SJ, Dhakal P, Zhang Z, Machacek M, Zachara NE, Koestler DC, Peterson KR, Thyfault JP, Swerdlow RH, Krishnamurthy P, DiTacchio L, Apte U, Slawson C. Sustained O-GlcNAcylation reprograms mitochondrial function to regulate energy metabolism. J Biol. Chem. 2017; 292(36):14940-14962. PMID: 28739801

Groves JA, Zachara NE. Characterization of tools to detect and enrich human and mouse O-GlcNAcase. Glycobiology. 2017 Jun 8;27(9):791–5. doi: 10.1093/glycob/cwx051. Epub ahead of print. PMID: 28595377; PMCID: PMC5881776.

Groves JA, Maduka AO, O’Meally RN, Cole RN, and Zachara NE.. Fatty acid synthase: a novel oxidative stress-induced interactor and inhibitor of the O-GlcNAcase. JBC, 2017; 292(16):6493-6511. PMID: 28232487.

Martinez M, Dias T, Natov P, and Zachara NE. Stress-Induced O-GlcNAcylation, an Adaptive Process of Injured Cells. Biochemical Society Transactions, 2017; 45(1): 237-249. PMID: 28202678.

2016

Lee A, Henry R, Miller D, Paruchuri VDP, O’Meally R, Boronina T, Cole RN, Zachara NE. Combined Antibody/Lectin-Enrichment Identifies Extensive Changes in the O-GlcNAc Subproteome Upon Oxidative Stress, Journal of Proteome Research; 15(12):4318-4336. PMID: 27669760

Fahie K, Zachara NE. Molecular Functions of Glycoconjugates in Autophagy. J Mol Biol. 2016; 428(16):3305-3024. Review. PMID: 27345664

Taparra K, Tran PT, Zachara NE. Hijacking the Hexosamine Biosynthetic Pathway to Promote EMT-Mediated Neoplastic Phenotypes. Front Oncol. 2016 Apr 18;6:85. PMID:27148477

Hou CW, Mohanan V, Zachara NE, Grimes CL. Identification and biological consequences of the O-GlcNAc modification of the human innate immune receptor, Nod2. Glycobiology. 2016; 26(1):13-18. PMID: 26369908.

2015

Zhu Y, Liu TW, Madden Z, Yuzwa SA, Murray K, Cecioni S, Zachara N, Vocadlo DJ. (2015) Post-translational O-GlcNAcylation is essential for nuclear pore integrity and maintenance of the pore selectivity filter. J Mol Cell Biol., 2015; 8(1):2-16. PMID: 26031751.

Zhong J, Martinez M, Sengupta S, Lee A, Wu X, Chaerkady R, Chatterjee A, O'Meally RN, Cole RN, Pandey A, Zachara NE. Quantitative phosphoproteomics reveals crosstalk between phosphorylation and O-GlcNAc in the DNA damage response pathway. Proteomics. 2015 Jan;15(2-3):591-607. doi: 10.1002/pmic.201400339. PMID: 25263469; PMCID: PMC4564869.

2014

Reeves RA, Lee A, Henry R, Zachara NE. Characterization of the specificity of O-GlcNAc reactive antibodies under conditions of starvation and stress. Anal Biochem. 2014 Jul 15;457:8-18. doi: 10.1016/j.ab.2014.04.008. Epub 2014 Apr 18. PMID: 24747005; PMCID: PMC4113912.

*Tardio L, *Andrés-Bergós J, Zachara NE, Larrañaga-Vera A, Rodriguez-Villar C, Herrero-Beaumont G, Largo R. O-linked N-acetylglucosamine (O-GlcNAc) protein modification is increased in the cartilage of patients with knee osteoarthritis. Osteoarthritis Cartilage. 2014 Feb;22(2):259-63. doi: 10.1016/j.joca.2013.12.001. Epub 2013 Dec 12. PMID: 24333294. *Denotes Co-First Authors

2013

Groves JA, Lee A, Yildirir G, Zachara NE. Dynamic O-GlcNAcylation and its roles in the cellular stress response and homeostasis. Cell Stress Chaperones. 2013 Sep;18(5):535-58. doi: 10.1007/s12192-013-0426-y. Epub 2013 Apr 26. PMID: 23620203; PMCID: PMC3745259.

Vibjerg Jensen R, Johnsen J, Buus Kristiansen S, Zachara NE, Bøtker HE. Ischemic preconditioning increases myocardial O-GlcNAc glycosylation. Scand Cardiovasc J. 2013 Jun;47(3):168-74. doi: 10.3109/14017431.2012.756984. Epub 2013 Jan 10. PMID: 23301939.

Jensen RV, Zachara NE, Nielsen PH, Kimose HH, Kristiansen SB, Bøtker HE. Impact of O-GlcNAc on cardioprotection by remote ischaemic preconditioning in non-diabetic and diabetic patients. Cardiovasc Res. 2013 Feb 1;97(2):369-78. doi: 10.1093/cvr/cvs337. Epub 2012 Dec 1. PMID: 23201773; PMCID: PMC3584969.

2012

Zachara NE. The roles of O-linked β-N-acetylglucosamine in cardiovascular physiology and disease. Am J Physiol Heart Circ Physiol. 2012 May 15;302(10):H1905-18. doi: 10.1152/ajpheart.00445.2011. Epub 2012 Jan 27. PMID: 22287582; PMCID: PMC3362101.

Tarrant MK, Rho HS, Xie Z, Jiang YL, Gross C, Culhane JC, Yan G, Qian J, Ichikawa Y, Matsuoka T, Zachara N, Etzkorn FA, Hart GW, Jeong JS, Blackshaw S, Zhu H, Cole PA. Regulation of CK2 by phosphorylation and O-GlcNAcylation revealed by semisynthesis. Nat Chem Biol. 2012 Jan 22;8(3):262-9. doi: 10.1038/nchembio.771. PMID: 22267120; PMCID: PMC3288285.

2011

Paruchuri VD, Zachara NE. Defining the heart and cardiovascular O-GlcNAcome: a review of approaches and methods. Circ Cardiovasc Genet. 2011 Dec;4(6):710. doi: 10.1161/CIRCGENETICS.110.957779. PMID: 22187449.

Zachara NE, Vosseller K, Hart GW. Detection and analysis of proteins modified by O-linked N-acetylglucosamine. Curr Protoc Protein Sci. 2011 Nov;Chapter 12:12.8.1-12.8.33. doi: 10.1002/0471140864.ps1208s66. PMID: 22045558; PMCID: PMC3349994.

Zachara NE, Vosseller K, Hart GW. Detection and analysis of proteins modified by O-linked N-acetylglucosamine. Curr Protoc Mol Biol. 2011 Jul;Chapter 17:Unit 17.6. doi: 10.1002/0471142727.mb1706s95. PMID: 21732316; PMCID: PMC3329785.

2010

Kazemi Z, Chang H, Haserodt S, McKen C, Zachara NE. O-linked beta-N-acetylglucosamine (O-GlcNAc) regulates stress-induced heat shock protein expression in a GSK-3beta-dependent manner. J Biol Chem. 2010 Dec 10;285(50):39096-107. doi: 10.1074/jbc.M110.131102. Epub 2010 Oct 6. PMID: 20926391; PMCID: PMC2998145.

Zachara NE*, Molina H, Wong KY, Pandey A, Hart GW*. The dynamic stress-induced "O-GlcNAc-ome" highlights functions for O-GlcNAc in regulating DNA damage/repair and other cellular pathways. Amino Acids. 2011 Mar;40(3):793-808. doi: 10.1007/s00726-010-0695-z. Epub 2010 Jul 31. PMID: 20676906; PMCID: PMC3329784. *Co-senior author

2009

Zachara NE. Detecting the "O-GlcNAc-ome"; detection, purification, and analysis of O-GlcNAc modified proteins. Methods Mol Biol. 2009;534:251-79. doi: 10.1007/978-1-59745-022-5_19. PMID: 19277546.

Ngoh GA, Facundo HT, Hamid T, Dillmann W, Zachara NE, Jones SP. Unique hexosaminidase reduces metabolic survival signal and sensitizes cardiac myocytes to hypoxia/reoxygenation injury. Circ Res. 2009 Jan 2;104(1):41-9. doi: 10.1161/CIRCRESAHA.108.189431. Epub 2008 Nov 20. PMID: 19023128; PMCID: PMC2712829.

Zachara NE. Detection and analysis of (O-linked beta-N-acetylglucosamine)-modified proteins. Methods Mol Biol. 2009;464:227-54. doi: 10.1007/978-1-60327-461-6_13. PMID: 18951188.

2008

Jones SP, Zachara NE, Ngoh GA, Hill BG, Teshima Y, Bhatnagar A, Hart GW, Marbán E. Cardioprotection by N-acetylglucosamine linkage to cellular proteins. Circulation. 2008 Mar 4;117(9):1172-82. doi: 10.1161/CIRCULATIONAHA.107.730515. Epub 2008 Feb 19. PMID: 18285568.

2007

Zachara NE. The sweet nature of cardioprotection. Am J Physiol Heart Circ Physiol. 2007 Sep;293(3):H1324-6. doi: 10.1152/ajpheart.00697.2007. Epub 2007 Jun 22. PMID: 17586610.

2006

Zachara NE, Hart GW. Cell signaling, the essential role of O-GlcNAc! Biochim Biophys Acta. 2006 May-Jun;1761(5-6):599-617. doi: 10.1016/j.bbalip.2006.04.007. Epub 2006 May 6. PMID: 16781888.

2005

Slawson C, Zachara NE, Vosseller K, Cheung WD, Lane MD, Hart GW. Perturbations in O-linked beta-N-acetylglucosamine protein modification cause severe defects in mitotic progression and cytokinesis. J Biol Chem. 2005 Sep 23;280(38):32944-56. doi: 10.1074/jbc.M503396200. Epub 2005 Jul 18. PMID: 16027160.

2004

Zachara NE, Cheung WD, Hart GW. Nucleocytoplasmic glycosylation, O-GlcNAc: identification and site mapping. Methods Mol Biol. 2004;284:175-94. doi: 10.1385/1-59259-816-1:175. PMID: 15173616.

Zachara NE, O'Donnell N, Cheung WD, Mercer JJ, Marth JD, Hart GW. Dynamic O-GlcNAc modification of nucleocytoplasmic proteins in response to stress. A survival response of mammalian cells. J Biol Chem. 2004 Jul 16;279(29):30133-42. doi: 10.1074/jbc.M403773200. Epub 2004 May 11. PMID: 15138254.

Zachara NE, Hart GW. O-GlcNAc modification: a nutritional sensor that modulates proteasome function. Trends Cell Biol. 2004 May;14(5):218-21. doi: 10.1016/j.tcb.2004.03.005. PMID: 15130576.

Zachara NE, Hart GW. Protein Glycosylation, Overview. In The Encyclopedia of Biochemistry. Lennarz W and Lande MD (Ed). Science Direct (New York). 2004; 3: 504-509.

Zachara NE, Hart GW. O-GlcNAc a sensor of cellular state: the role of nucleocytoplasmic glycosylation in modulating cellular function in response to nutrition and stress. Biochim Biophys Acta. 2004 Jul 6;1673(1-2):13-28. doi: 10.1016/j.bbagen.2004.03.016. PMID: 15238246.

Zachara NE, Cheung WD, Hart GW. Nucleocytoplasmic Glycosylation, O-linked β-N-Acetylglucosamine. Current Organic Chemistry. 2004; 8(5):369-83.
DOI: 10.2174/1385272043485873

O'Donnell N, Zachara NE, Hart GW, Marth JD. Ogt-dependent X-chromosome-linked protein glycosylation is a requisite modification in somatic cell function and embryo viability. Mol Cell Biol. 2004 Feb;24(4):1680-90. doi: 10.1128/MCB.24.4.1680-1690.2004. PMID: 14749383; PMCID: PMC344186.

Gewinner C, Hart G, Zachara N, Cole R, Beisenherz-Huss C, Groner B. The coactivator of transcription CREB-binding protein interacts preferentially with the glycosylated form of Stat5. J Biol Chem. 2004 Jan 30;279(5):3563-72. doi: 10.1074/jbc.M306449200. Epub 2003 Nov 3. PMID: 14597631.

2002

Zachara NE, Hart GW. The emerging significance of O-GlcNAc in cellular regulation. Chem Rev. 2002 Feb;102(2):431-8. doi: 10.1021/cr000406u. PMID: 11841249.

Zachara NE, Hart GW, Cole RN, Gao Y. Detection and analysis of proteins modified by O-linked N-acetylglucosamine. Curr Protoc Mol Biol. 2002 Feb;Chapter 17:Unit 17.6. doi: 10.1002/0471142727.mb1706s57. PMID: 18265305.

2001

Zachara NE, Cole RN, Hart GW, Gao Y. Detection and analysis of proteins modified by O-linked N-acetylglucosamine. Curr Protoc Protein Sci. 2001 Nov;Chapter 12:Unit 12.8. doi: 10.1002/0471140864.ps1208s25. PMID: 18429112.

2000

Zachara NE, Gooley AA. Identification of glycosylation sites in mucin peptides by edman degradation. Methods Mol Biol. 2000;125:121-8. doi: 10.1385/1-59259-048-9:121. PMID: 10820755.

1999

Wugeditsch T, Zachara NE, Puchberger M, Kosma P, Gooley AA, Messner P. Structural heterogeneity in the core oligosaccharide of the S-layer glycoprotein from Aneurinibacillus thermoaerophilus DSM 10155. Glycobiology. 1999 Aug;9(8):787-95. doi: 10.1093/glycob/9.8.787. PMID: 10406844.

Müller S, Alving K, Peter-Katalinic J, Zachara N, Gooley AA, Hanisch FG. High density O-glycosylation on tandem repeat peptide from secretory MUC1 of T47D breast cancer cells. J Biol Chem. 1999 Jun 25;274(26):18165-72. doi: 10.1074/jbc.274.26.18165. PMID: 10373415.

Hanisch FG, Müller S, Hassan H, Clausen H, Zachara N, Gooley AA, Paulsen H, Alving K, Peter-Katalinic J. Dynamic epigenetic regulation of initial O-glycosylation by UDP-N-Acetylgalactosamine:Peptide N-acetylgalactosaminyltransferases. site-specific glycosylation of MUC1 repeat peptide influences the substrate qualities at adjacent or distant Ser/Thr positions. J Biol Chem. 1999 Apr 9;274(15):9946-54. doi: 10.1074/jbc.274.15.9946. PMID: 10187769.

1998

Harrison MJ, Nouwens AS, Jardine DR, Zachara NE, Gooley AA, Nevalainen H, Packer NH. Modified glycosylation of cellobiohydrolase I from a high cellulase-producing mutant strain of Trichoderma reesei. Eur J Biochem. 1998 Aug 15;256(1):119-27. doi: 10.1046/j.1432-1327.1998.2560119.x. PMID: 9746354.

1997

Nevalainen H, Harrison M, Jardine D, Zachara NE, Paloheimo M, Suominen P, Gooley AA, and Packer P. (1997) Glycosylation of cellobiohydrolases from Trichoderma reesei. In Carbohydrates from Trichoderma reesei and other microorgansims: structure, biochemistry, genetics and applications. The Royal Society of Chemistry, Thomas Graham House, Cambridge, UK. pp 335-344.

1996

Zachara NE, Packer NH, Temple MD, Slade MB, Jardine DR, Karuso P, Moss CJ, Mabbutt BC, Curmi PM, Williams KL, Gooley AA. Recombinant prespore-specific antigen from Dictyostelium discoideum is a beta-sheet glycoprotein with a spacer peptide modified by O-linked N-acetylglucosamine. Eur J Biochem. 1996 Jun 1;238(2):511-8. doi: 10.1111/j.1432-1033.1996.0511z.x. PMID: 8681966.